HPLĆAPCÍMS Determination of Free Fatty Acids in Tibet Folk Medicine Lomatogonium rotatum with Fluorescence Detection and Mass Spectrometric Identification
Journal of Liquid Chromatography & Related Technologies
Format: Journal Article
Publication Year: 2006
Pages: 2741 - 2751
Source ID: shanti-sources-104001
Collection: Himalayan and Tibetan Medicine
Abstract: A simple and sensitive method for the determination of free fatty acids (FFAs) using acridoné9́ethyĺṕtoluenesulfonate (AETS) as a fluorescence derivatization reagent by high performance liquid chromatography (HPLC) has been developed. Free fatty acid derivatives were separated on an Eclipse XDB́C<sub>8</sub> column with a good baseline resolution and detected with the fluorescence of which excitation and emission wavelengths of derivatives were set at <sub>ex</sub>=404 and <sub>em</sub>=440 nm, respectively. Identification of 19 fatty acid derivatives was carried out by online post́column mass spectrometry with an atmospheric pressure chemical ionization (APCI) source under positivéion detection mode. Nineteen FFAs from the extract of <i>Lomatogonium rotatum</i> are sensitively determined. The results indicate that the plant <i>Lomatogonium rotatum</i> is enriched with an abundance of FFAs and FFAs of higher contents, which mainly focus on even carbon atoms, C<sub>14</sub>, C<sub>16</sub>, and C<sub>18</sub>. The validation of the method including linearity, repeatability, and detection limits was examined. Most linear correlation coefficients for fatty acid derivatives are >0.9989, and detection limits (at signaĺtónoise of 3:1) are 12.3-43.7 fmol. The relative standard deviations (RSDs) of the peak areas and retention times for 19 FFAs standards are <2.24% and 0.45%, respectively. The established method is rapid and reproducible for the separation determination of FFAs from the extract of <i>Lomatogonium rotatum</i> with satisfactory results.