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On-line HPLC-DPPH bioactivity-guided assay for isolated of antioxidative phenylpropanoids from Qinghai-Tibet Plateau medicinal plant Lancea tibetica
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
Short Title: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
Format: Journal Article
Publication Year: 2019
Pages: 1 - 10
Sources ID: 93656
Notes: Accession Number: 134204889; Wang, Weidong 1 Jiao, Lijin 1 Tao, Yanduo 1 Shao, Yun 1 Wang, Qilan 1 Yu, Ruitao 1 Mei, Lijuan 1; Email Address: meilijuan11@126.com Dang, Jun 1; Email Address: dangjun@nwipb.cas.cn; Affiliation:  1: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, China; Source Info: Feb2019, Vol. 1106, p1; Subject Term: HIGH performance liquid chromatography; Subject Term: PHENYLPROPANOIDS; Subject Term: MEDICINAL plants; Subject Term: ANTIOXIDANTS; Subject Term: BIOLOGICAL assay; Author-Supplied Keyword: Antioxidants; Author-Supplied Keyword: HPLC-DPPH; Author-Supplied Keyword: Lancea tibetica; Author-Supplied Keyword: Phenylpropanoids; Author-Supplied Keyword: Two-dimensional; Number of Pages: 10p; Document Type: Article; Full Text Word Count: 6951
Visibility: Public (group default)
Abstract: (Show)
Abstract Lancea tibetica is an important traditional Tibetan medicinal plant that grows on the Qinghai-Tibet Plateau with great development potential in pharmaceutical industry. In this study, a combinative method using HPLC-DPPH and two-dimensional liquid chromatography has been developed to identify and separate antioxidants from Lancea tibetica. Under the target-guidance of HPLC-DPPH experiment, three antioxidant fractions from Lancea tibetica were recognized. Then, separation of the three fractions using two-dimensional semi-preparation liquid chromatography led to seven phenylpropanoids: (+)-pinoresinol-β-D-glucoside (1), isoacteoside (2), acteoside (3), tibeticoside (4),epipinoresinol (5), anthelminthicol (6) and phillygenol (7). As a result, seven major antioxidants in Lancea tibetica were isolated with more than 96% purity. Furthermore, in vitro bioassay against DPPH revealed compounds 1 – 7 with IC 50 values ranging from 6.16 ± 0.08 to 25.09 ± 0.11 (μM) and compounds 1 , 2 and 3 showed activities stronger than the two reference antioxidants (vitamin C, rutin), with IC 50 values of 6.16 ± 0.08, 8.93 ± 0.06 and 7.98 ± 0.05 (μM), respectively. Results of the present study indicated that the method was an efficient technique to systematically screen and isolate antioxidants from medicine crops. Graphical abstract Unlabelled Image Highlights • A novel screen and separation method for purification of antioxidants directly. • Seven antioxidants isolated from Lancea tibetica bioactivity-guided. • Isolated antioxidants with IC 50 values from 6.16 ± 0.08 to 25.09 ± 0.11 (μM). • The first report on antioxidant capacity of the compounds from Lancea tibetica.