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Simultaneous determination of ten compounds in two main medicinal plant parts of Tibetan herb, Pterocephalus hookeri (CB Clarke) Höeck, by ultra-high performance liquid chromatography-photodiode array
Tropical Journal of Pharmaceutical Research
Short Title: Tropical Journal of Pharmaceutical Research
Format: Journal Article
Publication Date: 2017/06//
Pages: 1407 - 1416
Sources ID: 93666
Notes: Accession Number: 123946475; Ce Tang 1 Gang Fan 1 Qi Li 1 Jin-Song Su 1 Xian-Li Meng 1 Yi Zhang 1; Email Address:; Affiliation:  1: College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Source Info: Jun2017, Vol. 16 Issue 6, p1407; Subject Term: MEDICINAL plants; Subject Term: HERBAL medicine; Subject Term: CAPRIFOLIACEAE; Subject Term: HIGH performance liquid chromatography; Subject Term: PHOTODIODES; Author-Supplied Keyword: Loganic acid and Cantleyoside; Author-Supplied Keyword: Medicinal parts; Author-Supplied Keyword: Pterocephalus hookeri; Author-Supplied Keyword: Tibetan medicine; Author-Supplied Keyword: UPLC-PDA; Number of Pages: 10p; Document Type: Article
Visibility: Public (group default)
Abstract: (Show)
Purpose: To develop an ultra-high performance liquid chromatography (UPLC) - photodiode array (PDA) method to compare the chemical composition of two different medicinal components of Pterocephalus hookeri. Methods: Samples were chromatographically separated in succession using Waters Acquity UPLCR BEH C18 column (2.1 × 100 mm, 1.7 µm) and gradient elution (0.2% phosphoric acid aqueous - acetonitrile). Using partial least squares discriminant analysis and one-way analysis of variance, attempts were made to distinguish different medicinal parts of P. hookeri. Results: Regression equation for 10 compounds showed good linear regression (R² > 0.9994). The relative standard deviations of precision, stability, repeatability and recovery were under 5%. Compared with the aerial plant part, the root had significantly higher levels of sylvestroside I (p < 0.01), cantleyoside (p < 0.001), dipsanosides B (p < 0.01) and dipsanosides A (p < 0.01), but significantly lower levels of loganic acid (p < 0.001), chlorogenic acid (p < 0.01), and isochlorogenic acid (p < 0.01). There were no significant differences between loganin, sweroside and isochlorogenic acid C. Conclusion: The described method is simple, accurate and reproducible, and can be used for the simultaneous determination of 10 major compounds of P. hookeri. The results demonstrate that there is variation in the chemical composition of the aerialpart and root of P. hookeri and that loganic acid and cantleyoside are the primary chemical biomarkers.