A simple, efficient and accurate liquid chromatographic method was established to determine five flavonoid aglycones, 7-hydroxy flavonone, pinocembrin, 2′,4′-dihydroxy chalcone, 2′-hydroxy-4′-methoxy chalcone and pinostrobin in the whole plant powder of Oxytropis falcata Bunge. These five compounds were separated on an Agilent Zorbax Eclipse XDB-C₈ column (150 × 4.6 mm, 5 μm). Mobile phases were composed of water containing 0.1% v/v formic acid and acetonitrile using gradient elution. The established method was validated for linearity, accuracy, precision, limit of detection and quantitation, repeatability and stability.