Triphala Suppresses Growth and Migration of Human Gastric Carcinoma Cells In Vitro and in a Zebrafish Xenograft Model.
BioMed Research International
Short Title:
BioMed Research International
Format:
Journal Article
Publication Date:
2018/12/10/
Pages:
1 - 6
Sources ID:
93336
Notes:
Accession Number: 133473422; Tsering, Jokyab 1 Hu, Xianda 1; Affiliation: 1: Beijing Tibetan Hospital, China Tibetology Research Center, Beijing, China; Source Info: 12/10/2018, p1; Subject Term: CELL proliferation; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL assay; Subject Term: BIOLOGICAL models; Subject Term: CELL lines; Subject Term: CELL receptors; Subject Term: CELL motility; Subject Term: CELLULAR signal transduction; Subject Term: CHOLECYSTOKININ; Subject Term: EPIDERMAL growth factor; Subject Term: FISHES; Subject Term: MEDICINAL plants; Subject Term: METASTASIS; Subject Term: PHOSPHORYLATION; Subject Term: PROTEIN kinases; Subject Term: STOMACH; Subject Term: STOMACH tumors; Subject Term: TUMOR classification; Subject Term: WESTERN immunoblotting; Subject Term: XENOGRAFTS; Subject Term: PLANT extracts; Subject Term: IN vitro studies; Subject Term: IN vivo studies; Number of Pages: 6p; Document Type: Article; Full Text Word Count: 3515Accession Number: 133473422; Tsering, Jokyab 1 Hu, Xianda 1; Affiliation: 1: Beijing Tibetan Hospital, China Tibetology Research Center, Beijing, China; Source Info: 12/10/2018, p1; Subject Term: CELL proliferation; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL assay; Subject Term: BIOLOGICAL models; Subject Term: CELL lines; Subject Term: CELL receptors; Subject Term: CELL motility; Subject Term: CELLULAR signal transduction; Subject Term: CHOLECYSTOKININ; Subject Term: EPIDERMAL growth factor; Subject Term: FISHES; Subject Term: MEDICINAL plants; Subject Term: METASTASIS; Subject Term: PHOSPHORYLATION; Subject Term: PROTEIN kinases; Subject Term: STOMACH; Subject Term: STOMACH tumors; Subject Term: TUMOR classification; Subject Term: WESTERN immunoblotting; Subject Term: XENOGRAFTS; Subject Term: PLANT extracts; Subject Term: IN vitro studies; Subject Term: IN vivo studies; Number of Pages: 6p; Document Type: Article; Full Text Word Count: 3515Accession Number: 133473422; Tsering, Jokyab 1 Hu, Xianda 1; Affiliation: 1: Beijing Tibetan Hospital, China Tibetology Research Center, Beijing, China; Source Info: 12/10/2018, p1; Subject Term: CELL proliferation; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL assay; Subject Term: BIOLOGICAL models; Subject Term: CELL lines; Subject Term: CELL receptors; Subject Term: CELL motility; Subject Term: CELLULAR signal transduction; Subject Term: CHOLECYSTOKININ; Subject Term: EPIDERMAL growth factor; Subject Term: FISHES; Subject Term: MEDICINAL plants; Subject Term: METASTASIS; Subject Term: PHOSPHORYLATION; Subject Term: PROTEIN kinases; Subject Term: STOMACH; Subject Term: STOMACH tumors; Subject Term: TUMOR classification; Subject Term: WESTERN immunoblotting; Subject Term: XENOGRAFTS; Subject Term: PLANT extracts; Subject Term: IN vitro studies; Subject Term: IN vivo studies; Number of Pages: 6p; Document Type: Article; Full Text Word Count: 3515
Collection:
Himalayan and Tibetan Medicine
Visibility:
Public (group default)
Abstract:
(Show)
Objectives. Triphala is an extensively prescribed traditional medicinal formula with potential therapeutic effects on various malignancies such as breast, colon, pancreas, prostate, ovarian, cervical, endometrial, and lymphatic cancer as well as melanoma. This study aimed to investigate Triphala for antitumor activities against gastric cancers. Methods. In vitro tumor growth and migration of human gastric cancer cells were examined using the CCK-8 and Transwell assays, respectively. In vivo tumor progression was studied in a zebrafish xenograft model. The anticancer activity of Triphala was quantified as growth and metastasis inhibition rate. The underlying molecular mechanism was investigated by Western blotting. Results. The CCK-8 and Transwell experiments indicated that Triphala significantly decreased tumor proliferation and suppressed cell migration in vitro. The zebrafish xenograft study revealed that administration of Triphala inhibited the xenograft growth and metastasis of transplanted carcinoma cells in vivo. Western blotting analysis demonstrated an inhibition of phosphorylation of EGFR, Akt, and ERK in the presence of Triphala, indicating that its antineoplastic mechanism is associated with the regulation of the EGFR/Akt/ERK signaling cascade. Conclusion. Triphala is a promising antineoplastic agent for the treatment of gastric carcinomas with significant antiproliferative and antimetastatic activities. [ABSTRACT FROM AUTHOR]