Study on anti-hyperuricemia effects and active ingredients of traditional Tibetan medicine TongFengTangSan (TFTS) by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
Journal of Pharmaceutical & Biomedical Analysis
Short Title:
Journal of Pharmaceutical & Biomedical Analysis
Format:
Journal Article
Publication Date:
2019/02/20/
Pages:
213 - 223
Sources ID:
93366
Notes:
Accession Number: 134069033; Chen, Hai-fang 1 Zhang, Chen 1 Yao, Yun 1 Li, Jun-mao 1 Du, Wen-di 1 Li, Mu-lan 1 Wu, Bei 2 Yang, Shi-lin 1 Feng, Yu-lin 1; Email Address: fengyulin2003@126.com Zhang, Wu-gang 1; Email Address: Zwgchf98@foxmail.com; Affiliation: 1: Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Road, Nanchang, 330004, PR China 2: Nanchang Insitute for Food and Drug Control, No. 299 Diezihu Road, Nanchang, 330038, PR China; Source Info: Feb2019, Vol. 165, p213; Subject Term: HYPERURICEMIA; Subject Term: TIBETAN medicine; Subject Term: HIGH performance liquid chromatography; Subject Term: QUADRUPOLES; Subject Term: TIME-of-flight mass spectrometry; Subject Term: ETHANOL; Author-Supplied Keyword: Active ingredients; Author-Supplied Keyword: Anti-hyperuricemic effects; Author-Supplied Keyword: MS/MS cracking rule; Author-Supplied Keyword: TongFengTangSan; Author-Supplied Keyword: Traditional Tibetan medicine; Author-Supplied Keyword: UHPLC-Q-TOF-MS/MS; NAICS/Industry Codes: 312140 Distilleries; NAICS/Industry Codes: 325190 Other basic organic chemical manufacturing; NAICS/Industry Codes: 325193 Ethyl Alcohol Manufacturing; Number of Pages: 11p; Document Type: ArticleAccession Number: 134069033; Chen, Hai-fang 1 Zhang, Chen 1 Yao, Yun 1 Li, Jun-mao 1 Du, Wen-di 1 Li, Mu-lan 1 Wu, Bei 2 Yang, Shi-lin 1 Feng, Yu-lin 1; Email Address: fengyulin2003@126.com Zhang, Wu-gang 1; Email Address: Zwgchf98@foxmail.com; Affiliation: 1: Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Road, Nanchang, 330004, PR China 2: Nanchang Insitute for Food and Drug Control, No. 299 Diezihu Road, Nanchang, 330038, PR China; Source Info: Feb2019, Vol. 165, p213; Subject Term: HYPERURICEMIA; Subject Term: TIBETAN medicine; Subject Term: HIGH performance liquid chromatography; Subject Term: QUADRUPOLES; Subject Term: TIME-of-flight mass spectrometry; Subject Term: ETHANOL; Author-Supplied Keyword: Active ingredients; Author-Supplied Keyword: Anti-hyperuricemic effects; Author-Supplied Keyword: MS/MS cracking rule; Author-Supplied Keyword: TongFengTangSan; Author-Supplied Keyword: Traditional Tibetan medicine; Author-Supplied Keyword: UHPLC-Q-TOF-MS/MS; NAICS/Industry Codes: 312140 Distilleries; NAICS/Industry Codes: 325190 Other basic organic chemical manufacturing; NAICS/Industry Codes: 325193 Ethyl Alcohol Manufacturing; Number of Pages: 11p; Document Type: ArticleAccession Number: 134069033; Chen, Hai-fang 1 Zhang, Chen 1 Yao, Yun 1 Li, Jun-mao 1 Du, Wen-di 1 Li, Mu-lan 1 Wu, Bei 2 Yang, Shi-lin 1 Feng, Yu-lin 1; Email Address: fengyulin2003@126.com Zhang, Wu-gang 1; Email Address: Zwgchf98@foxmail.com; Affiliation: 1: Jiangxi University of Traditional Chinese Medicine, No. 1688 Meiling Road, Nanchang, 330004, PR China 2: Nanchang Insitute for Food and Drug Control, No. 299 Diezihu Road, Nanchang, 330038, PR China; Source Info: Feb2019, Vol. 165, p213; Subject Term: HYPERURICEMIA; Subject Term: TIBETAN medicine; Subject Term: HIGH performance liquid chromatography; Subject Term: QUADRUPOLES; Subject Term: TIME-of-flight mass spectrometry; Subject Term: ETHANOL; Author-Supplied Keyword: Active ingredients; Author-Supplied Keyword: Anti-hyperuricemic effects; Author-Supplied Keyword: MS/MS cracking rule; Author-Supplied Keyword: TongFengTangSan; Author-Supplied Keyword: Traditional Tibetan medicine; Author-Supplied Keyword: UHPLC-Q-TOF-MS/MS; NAICS/Industry Codes: 312140 Distilleries; NAICS/Industry Codes: 325190 Other basic organic chemical manufacturing; NAICS/Industry Codes: 325193 Ethyl Alcohol Manufacturing; Number of Pages: 11p; Document Type: Article
Collection:
Himalayan and Tibetan Medicine
Visibility:
Public (group default)
Abstract:
(Show)
Graphical abstract Highlights • The anti-hyperuricemia effects and active components of the traditional Tibetan medicine formula TFTS were investigated. • Total 106 compontents were identified or characterized in TFTS by UHPLC-Q-TOF-MS/MS. • It was summarized the diagnostic ion and neutral loss patterns of MS/MS cracking of tannic compounds. Abstract TongFengTangSan (TFTS), a traditional Tibetan medicine comprising of Tinospora sinensis (TS), Terminalia chebula Retz (TC) and Trogopterori faeces (TF), is used to treat joint diseases like gout, gout arthritis, swelling, pain etc. Despite the significant therapeutic effects of TFTS, its pharmacological components have not been analyzed so far. Therefore, the chemical composition of the effective part of TFTS was analyzed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). The results show that the ethanol extract (EE) of TFTS was more effective in reducing the serum uric acid (SUA) and XOD (Serum and Liver) levels in a hyperuricemic rats model compared to the TFTS raw powder (RP). UHPLC-Q-TOF-MS/MS identified a total of 106 compounds in the positive and negative ion mode, of which 87 were from TC, 13 from TS and 6 from TF. In addition, 106 compounds contained 57 tannins, 6 triterpenoids, 10 alkaloids, 7 flavonoids, 22 organic acids and 4 phenylpropanoids. The preliminary results indicate that the EE of TFTS includes the active anti hyperuricemic substances. The present study first investigated the efficacy and the active components of TFTS in hyperuricemic treatment, and further summarized the diagnostic ion and neutral loss patterns of MS/MS cracking of tannic compounds. These findings lay the foundation for the further study and clinical application of TFTS. [ABSTRACT FROM AUTHOR]