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Callus Induction and Rapid Propagation Systems from a Tibetan Medicine Plant, Veronica ciliate Fisch
Plant Science Journal
Short Title: Plant Science Journal
Format: Journal Article
Publication Date: 2011/08/01/
Pages: 531 - 535
Sources ID: 95711
Visibility: Public (group default)
Abstract: (Show)
To induce callus and establish rapid propagation systems for Veronica ciliate Fisch., terminal buds from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA. Results showed that the optimum medium for callus inducement was MS + 6-BA 0. 5 mg/L + NAA 1. 0 mg/L, with the callus induction rate of 95%. The best medium for multiplication was MS + 6-BA 0. 5 mg/L + NAA 0. 1 mg/L,with the multiplication coefficient reaching 5. 4. The optimum root medium for multiple shoot clumps was 1/2 MS + IBA 0. 05 mg/L,with the rooting rate reaching 95% and the root being healthiest. Under suitable hardening and transplanting conditions, the highest survival rate for the regenerated plantlets reached 40%.