Callus Induction and Rapid Propagation Systems from a Tibetan Medicine Plant, Veronica ciliate Fisch
Plant Science Journal
Short Title:
Plant Science Journal
Format:
Journal Article
Publication Date:
2011/08/01/
Pages:
531 - 535
Sources ID:
95711
Collection:
Himalayan and Tibetan Medicine
Visibility:
Public (group default)
Abstract:
(Show)
To induce callus and establish rapid propagation systems for Veronica ciliate Fisch., terminal buds from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA. Results showed that the optimum medium for callus inducement was MS + 6-BA 0. 5 mg/L + NAA 1. 0 mg/L, with the callus induction rate of 95%. The best medium for multiplication was MS + 6-BA 0. 5 mg/L + NAA 0. 1 mg/L,with the multiplication coefficient reaching 5. 4. The optimum root medium for multiple shoot clumps was 1/2 MS + IBA 0. 05 mg/L,with the rooting rate reaching 95% and the root being healthiest. Under suitable hardening and transplanting conditions, the highest survival rate for the regenerated plantlets reached 40%.