Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats
Biomedical Reports
Short Title:
Biomedical Reports
Format:
Journal Article
Publication Date:
2016/02//
Pages:
161 - 166
Sources ID:
93636
Notes:
Accession Number: 112385789; TIAN WANG 1 SIJIN DUAN 1 HAIPING WANG 2 SHAN SUN 2 BING HAN 3 FENGHUA FU 1; Email Address: fufenghua@sohu.com; Affiliation: 1: Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China 2: Shandong ARURA Pharmaceutical R&D Co., Ltd., Jinan, Shandong 250101, P.R. China 3: Department of Food Safety, School of Life Science, Yantai University, Yantai, Shandong 264005, P.R. China; Source Info: Feb2016, Vol. 4 Issue 2, p161; Subject Term: CEREBRAL ischemia; Subject Term: REPERFUSION injury; Subject Term: TIBETAN medicine; Subject Term: NEUROLOGICAL research; Subject Term: SODIUM carboxymethyl cellulose; Subject Term: BRAIN-derived neurotrophic factor; Subject Term: NERVE Growth Factor; Subject Term: VASCULAR endothelial growth factors; Author-Supplied Keyword: angiogenesis; Author-Supplied Keyword: cerebral ischemia; Author-Supplied Keyword: neurogenesis; Author-Supplied Keyword: neurological function; Author-Supplied Keyword: Ruyi Zhenbao pill; Number of Pages: 6p; Document Type: Article
Collection:
Himalayan and Tibetan Medicine
Visibility:
Public (group default)
Abstract:
(Show)
The present study aimed to investigate the effect and underlying mechanisms of the Ruyi Zhenbao pill on neurological function following cerebral ischemia/reperfusion in rats. Male Sprague-Dawley rats underwent middle cerebral artery occlusion following reperfusion. The rats received intragastrically either sodium carboxymethyl cellulose (control and model groups) or Ruyi Zhenbao pill at doses of 0.2, 0.4 or 0.8 g/kg. Neurological function was assessed by cylinder, adhesive and beam-walking tests after 14-day Ruyi Zhenbao pill treatment. Neurogenesis and angiogenesis were detected using immunofluorescence staining. The expression levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assays. Treatment with 0.4 and 0.8 g/kg Ruyi Zhenbao for 14 days significantly improved neurological function, and increased the number of von Willebrand Factor- and neuronal nuclear antigen-positive cells in the ischemic hemisphere of rats. Ruyi Zhenbao pill treatment also significantly enhanced the expression levels of BDNF, NGF and VEGF in the ischemic hemisphere. The results demonstrated that the Ruyi Zhenbao pill improved neurological function following ischemia in rats. The mechanisms of the Ruyi Zhenbao pill are associated with increasing the expression levels of BDNF, NGF and VEGF, and subsequently promoting neurogenesis and angiogenesis in the ischemic zone.