Compound Phyllanthus urinaria L Inhibits HBV-Related HCC through HBx-SHH Pathway Axis Inactivation.
Evidence-based Complementary & Alternative Medicine (eCAM)
Short Title:
Evidence-based Complementary & Alternative Medicine (eCAM)
Format:
Journal Article
Publication Date:
2019/03/24/
Pages:
1 - 15
Sources ID:
93451
Notes:
Accession Number: 135508328; Source Information: 3/24/2019, p1; Subject Term: PROTEIN metabolism; Subject Term: CELL proliferation; Subject Term: ANIMAL experimentation; Subject Term: ANTINEOPLASTIC agents; Subject Term: CELL motility; Subject Term: CELLULAR signal transduction; Subject Term: DOSE-effect relationship in pharmacology; Subject Term: GENE expression; Subject Term: GENES; Subject Term: GENETIC techniques; Subject Term: HEPATITIS B; Subject Term: HEPATOMA; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: MOLECULAR structure; Subject Term: TRANSCRIPTION factors; Subject Term: PLANT extracts; Subject Term: IN vitro studies; Subject Term: TUMOR grading; Subject Term: PHARMACODYNAMICS; Subject Term: ; Number of Pages: 15p; ; Illustrations: 1 Diagram, 1 Chart, 9 Graphs; ; Document Type: Article; ; Full Text Word Count: 5637;Accession Number: 135508328; Source Information: 3/24/2019, p1; Subject Term: PROTEIN metabolism; Subject Term: CELL proliferation; Subject Term: ANIMAL experimentation; Subject Term: ANTINEOPLASTIC agents; Subject Term: CELL motility; Subject Term: CELLULAR signal transduction; Subject Term: DOSE-effect relationship in pharmacology; Subject Term: GENE expression; Subject Term: GENES; Subject Term: GENETIC techniques; Subject Term: HEPATITIS B; Subject Term: HEPATOMA; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: MOLECULAR structure; Subject Term: TRANSCRIPTION factors; Subject Term: PLANT extracts; Subject Term: IN vitro studies; Subject Term: TUMOR grading; Subject Term: PHARMACODYNAMICS; Subject Term: ; Number of Pages: 15p; ; Illustrations: 1 Diagram, 1 Chart, 9 Graphs; ; Document Type: Article; ; Full Text Word Count: 5637;Accession Number: 135508328; Source Information: 3/24/2019, p1; Subject Term: PROTEIN metabolism; Subject Term: CELL proliferation; Subject Term: ANIMAL experimentation; Subject Term: ANTINEOPLASTIC agents; Subject Term: CELL motility; Subject Term: CELLULAR signal transduction; Subject Term: DOSE-effect relationship in pharmacology; Subject Term: GENE expression; Subject Term: GENES; Subject Term: GENETIC techniques; Subject Term: HEPATITIS B; Subject Term: HEPATOMA; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: MOLECULAR structure; Subject Term: TRANSCRIPTION factors; Subject Term: PLANT extracts; Subject Term: IN vitro studies; Subject Term: TUMOR grading; Subject Term: PHARMACODYNAMICS; Subject Term: ; Number of Pages: 15p; ; Illustrations: 1 Diagram, 1 Chart, 9 Graphs; ; Document Type: Article; ; Full Text Word Count: 5637;
Collection:
Himalayan and Tibetan Medicine
Visibility:
Public (group default)
Abstract:
(Show)
Compound Phyllanthus urinaria L (CP) is a traditional formula widely used in clinical practice for hepatocellular carcinoma (HCC), especially HBV-related HCC. HBx, HBV X gene encoded X protein, has positive correlation with the abnormal SHH pathway in HBV-related HCC. So, we predicted that CP has the capability of anti-HBV-related HCC maybe via inactivating the HBx-Hedgehog pathway axis. HepG2-HBx cells, HBx overexpression, were treated with CP (70μg/ml and 35 μg/ml, respectively) for 48 hours and the mice which received the HepG2-HBx cells were treated with CP (625mg/kg and 300 mg/kg, respectively) for 17 days to evaluate the effect of CP on HBV-related HCC. HBx could accelerate HepG2 cells proliferation, clone formation, and migration in vitro and also could strengthen tumor growth in mice. However, CP could significantly decrease HepG2-HBx cells proliferation, clone formation, and migration in vitro and also could inhibit tumors growth in mice in a dose-dependent manner. Mechanism studies suggested that HBx upregulated the mRNA and proteins expression of Sonic hedgehog (SHH), transmembrane receptor patched (PTCH-1), smoothened (SMO), oncogene homolog transcription factors-1 (GLI-1), and oncogene homolog transcription factors-2 (GLI-2), which are compositions of the SHH pathway. CP could inhibit the mRNA and proteins expression of SHH, PTCH-1, GLI-1, and HBx. It may be one of the underlying mechanisms of CP to delay the HBV-related HCC development through the HBx-SHH pathway axis inactivation. [ABSTRACT FROM AUTHOR]