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Traditional Tibetan medicine Anzhijinhua San attenuates ovalbumin-induced diarrhea by regulating the serotonin signaling system in mice.
Journal of Ethnopharmacology
Short Title: Journal of Ethnopharmacology
Format: Journal Article
Publication Date: 2019/05/23/
Pages: 484 - 494
Sources ID: 93341
Notes: Accession Number: 135686049; Xia, Zhenjiang 1,2,3,4; Email Address: yiuli1984@126.com Zhang, Yifan 3,5,6; Email Address: yfanv3@163.com Li, Cen 1,2,3; Email Address: licen_601@126.com Xu, Yan 3,5,6; Email Address: 18260090078@163.com Dong, Jinjin 3,5,6; Email Address: 874370512@qq.com Wang, Lulu 3,5,6; Email Address: wuyuzhilu@sina.com He, Qiangqiang 1,2,4; Email Address: 8075874@163.com Zou, Xiaoyan 1,2,4; Email Address: dayan2200@163.com Wu, Huali 3,5,6; Email Address: wu5254307@163.com Han, Jichun 3,5,6; Email Address: 923023681@qq.com Cai, Minxuan 3,5,6; Email Address: cmxc3h6@126.com Du, Yuzhi 1,2; Email Address: yzdu@nwipb.cas.cn Wei, Lixin 1,2,3; Email Address: lxwei@nwipb.cas.cn Shang, Jing 1,2,3,5,6; Email Address: shangjing21cn@cpu.edu.cn; Affiliation:  1: Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  2: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  3: School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  4: University of Chinese Academy of Sciences, Beijing 100049, China  5: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  6: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Source Info: May2019, Vol. 236, p484; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL models; Subject Term: CELLULAR signal transduction; Subject Term: DIARRHEA; Subject Term: GENE expression; Subject Term: HISTOLOGY; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: SEROTONIN; Subject Term: ALBUMINS; Author-Supplied Keyword: Allergic diarrhea; Author-Supplied Keyword: Anzhijinhua San; Author-Supplied Keyword: Ovalbumin; Author-Supplied Keyword: Serotonin; Author-Supplied Keyword: Tibetan medicine; NAICS/Industry Codes: 112990 All Other Animal Production; Number of Pages: 11p; Document Type: Article; Full Text Word Count: 6715Accession Number: 135686049; Xia, Zhenjiang 1,2,3,4; Email Address: yiuli1984@126.com Zhang, Yifan 3,5,6; Email Address: yfanv3@163.com Li, Cen 1,2,3; Email Address: licen_601@126.com Xu, Yan 3,5,6; Email Address: 18260090078@163.com Dong, Jinjin 3,5,6; Email Address: 874370512@qq.com Wang, Lulu 3,5,6; Email Address: wuyuzhilu@sina.com He, Qiangqiang 1,2,4; Email Address: 8075874@163.com Zou, Xiaoyan 1,2,4; Email Address: dayan2200@163.com Wu, Huali 3,5,6; Email Address: wu5254307@163.com Han, Jichun 3,5,6; Email Address: 923023681@qq.com Cai, Minxuan 3,5,6; Email Address: cmxc3h6@126.com Du, Yuzhi 1,2; Email Address: yzdu@nwipb.cas.cn Wei, Lixin 1,2,3; Email Address: lxwei@nwipb.cas.cn Shang, Jing 1,2,3,5,6; Email Address: shangjing21cn@cpu.edu.cn; Affiliation:  1: Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  2: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  3: School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  4: University of Chinese Academy of Sciences, Beijing 100049, China  5: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  6: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Source Info: May2019, Vol. 236, p484; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL models; Subject Term: CELLULAR signal transduction; Subject Term: DIARRHEA; Subject Term: GENE expression; Subject Term: HISTOLOGY; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: SEROTONIN; Subject Term: ALBUMINS; Author-Supplied Keyword: Allergic diarrhea; Author-Supplied Keyword: Anzhijinhua San; Author-Supplied Keyword: Ovalbumin; Author-Supplied Keyword: Serotonin; Author-Supplied Keyword: Tibetan medicine; NAICS/Industry Codes: 112990 All Other Animal Production; Number of Pages: 11p; Document Type: Article; Full Text Word Count: 6715Accession Number: 135686049; Xia, Zhenjiang 1,2,3,4; Email Address: yiuli1984@126.com Zhang, Yifan 3,5,6; Email Address: yfanv3@163.com Li, Cen 1,2,3; Email Address: licen_601@126.com Xu, Yan 3,5,6; Email Address: 18260090078@163.com Dong, Jinjin 3,5,6; Email Address: 874370512@qq.com Wang, Lulu 3,5,6; Email Address: wuyuzhilu@sina.com He, Qiangqiang 1,2,4; Email Address: 8075874@163.com Zou, Xiaoyan 1,2,4; Email Address: dayan2200@163.com Wu, Huali 3,5,6; Email Address: wu5254307@163.com Han, Jichun 3,5,6; Email Address: 923023681@qq.com Cai, Minxuan 3,5,6; Email Address: cmxc3h6@126.com Du, Yuzhi 1,2; Email Address: yzdu@nwipb.cas.cn Wei, Lixin 1,2,3; Email Address: lxwei@nwipb.cas.cn Shang, Jing 1,2,3,5,6; Email Address: shangjing21cn@cpu.edu.cn; Affiliation:  1: Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  2: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  3: School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  4: University of Chinese Academy of Sciences, Beijing 100049, China  5: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  6: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Source Info: May2019, Vol. 236, p484; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL models; Subject Term: CELLULAR signal transduction; Subject Term: DIARRHEA; Subject Term: GENE expression; Subject Term: HISTOLOGY; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: SEROTONIN; Subject Term: ALBUMINS; Author-Supplied Keyword: Allergic diarrhea; Author-Supplied Keyword: Anzhijinhua San; Author-Supplied Keyword: Ovalbumin; Author-Supplied Keyword: Serotonin; Author-Supplied Keyword: Tibetan medicine; NAICS/Industry Codes: 112990 All Other Animal Production; Number of Pages: 11p; Document Type: Article; Full Text Word Count: 6715Accession Number: 135686049; Xia, Zhenjiang 1,2,3,4; Email Address: yiuli1984@126.com Zhang, Yifan 3,5,6; Email Address: yfanv3@163.com Li, Cen 1,2,3; Email Address: licen_601@126.com Xu, Yan 3,5,6; Email Address: 18260090078@163.com Dong, Jinjin 3,5,6; Email Address: 874370512@qq.com Wang, Lulu 3,5,6; Email Address: wuyuzhilu@sina.com He, Qiangqiang 1,2,4; Email Address: 8075874@163.com Zou, Xiaoyan 1,2,4; Email Address: dayan2200@163.com Wu, Huali 3,5,6; Email Address: wu5254307@163.com Han, Jichun 3,5,6; Email Address: 923023681@qq.com Cai, Minxuan 3,5,6; Email Address: cmxc3h6@126.com Du, Yuzhi 1,2; Email Address: yzdu@nwipb.cas.cn Wei, Lixin 1,2,3; Email Address: lxwei@nwipb.cas.cn Shang, Jing 1,2,3,5,6; Email Address: shangjing21cn@cpu.edu.cn; Affiliation:  1: Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  2: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China  3: School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  4: University of Chinese Academy of Sciences, Beijing 100049, China  5: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 211198, China  6: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Source Info: May2019, Vol. 236, p484; Subject Term: ANIMAL experimentation; Subject Term: BIOLOGICAL models; Subject Term: CELLULAR signal transduction; Subject Term: DIARRHEA; Subject Term: GENE expression; Subject Term: HISTOLOGY; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MICE; Subject Term: SEROTONIN; Subject Term: ALBUMINS; Author-Supplied Keyword: Allergic diarrhea; Author-Supplied Keyword: Anzhijinhua San; Author-Supplied Keyword: Ovalbumin; Author-Supplied Keyword: Serotonin; Author-Supplied Keyword: Tibetan medicine; NAICS/Industry Codes: 112990 All Other Animal Production; Number of Pages: 11p; Document Type: Article; Full Text Word Count: 6715
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Abstract: (Show)
Abstract Ethnopharmacological relevance Tibetan medicine has been practiced for 3800 years. Anzhijinhua San (AZJHS), which is a traditional Tibetan medicine, has been effective in the treatment of indigestion, anorexia and cold diarrhea. However, the effects of AZJHS on allergic diarrhea have not been reported. Aim of the study The aim of the present study was to elucidate the effect of AZJHS on experimental ovalbumin-induced diarrhea and elucidate its possible mechanism. Materials and methods Female BALB/c mice were sensitized by intraperitoneal injection with 50 μg ovalbumin (OVA) and 1 mg alum in saline twice during a 2-week period. From day 28, mice were orally challenged with OVA (50 mg) every other day for a total of ten times. AZJHS (46.8 and 468.0 mg/kg) was orally administered every other day from day 0–46. Food allergy symptoms were evaluated. OVA- specific IgE, 5-HT and its metabolites in serum were determined. Immunohistochemical and histopathology were performed in gastrointestinal tract tissues. 5-HT-related gene expression was assayed in the colon. Results Severe symptoms of allergic diarrhea were observed in the model group (diarrhea, anaphylactic response, and rectal temperature). AZJHS (46.8 and 468.0 mg/kg) significantly reduced mouse diarrhea and significantly prevented the increases in OVA-specific IgE levels (P < 0.05), which challenge with OVA. AZJHS (46.8 and 468.0 mg/kg) significantly prevented the increases in 5-HT-positive cells. The nuclei of EC cells in the AZJHS (46.8 and 468.0 mg/kg) group increased in size and the secretory granules were fewer in number compared with those in the model group. AZJHS (46.8 and 468.0 mg/kg) significantly increased the relative fold changes of 5-HTP and 5-HT compared with the model group. The mRNA expression of the serotonin transporter (Sert) and serotonin receptor 3A (Htr3a) was significantly decreased after the 10th challenge with OVA, and AZJHS (46.8 and 468.0 mg/kg) significantly increased these levels. Conclusions We demonstrated that the administration of AZJHS attenuated OVA-induced diarrhea by regulating the serotonin pathway. These results indicated that AZJHS may be a potential candidate as an anti-allergic diarrhea agent. Graphical abstract fx1 [ABSTRACT FROM AUTHOR]