Srolo Bzhtang, a traditional Tibetan medicine formula, inhibits cigarette smoke induced airway inflammation and muc5ac hypersecretion via suppressing IL-13/STAT6 signaling pathway in rats.
Journal of Ethnopharmacology
Short Title:
Journal of Ethnopharmacology
Format:
Journal Article
Publication Date:
2019/05/10/
Pages:
424 - 434
Sources ID:
93371
Notes:
Accession Number: 135255914; Jing, Linde 1,2 Su, Shanshan 3 Zhang, Dejun 4 Li, Zhanqiang 1,2; Email Address: zhanqiang_li@163.com Lu, Dianxiang 1,2; Email Address: ludianxiang@qhu.edu.cn Ge, Rili 1,2; Email Address: geriligao@hotmail.com; Affiliation: 1: Research Center for High Altitude Medicine, Qinghai University, Xining 810001, China 2: Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province, Xining 810001, China 3: Xining Customs District, Key Laboratory of Food Safety Research in Qinghai Province, Xining 810003, China 4: School of Ecological and Environmental Engineering, Qinghai University, Xining 810016; Source Info: May2019, Vol. 235, p424; Subject Term: ANIMAL experimentation; Subject Term: BENZOPYRANS; Subject Term: BRONCHOALVEOLAR lavage; Subject Term: CELLULAR signal transduction; Subject Term: CYTOKINES; Subject Term: ENZYME-linked immunosorbent assay; Subject Term: GENE expression; Subject Term: GLYCOPROTEINS; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: INFLAMMATORY mediators; Subject Term: INTERLEUKINS; Subject Term: LUNGS; Subject Term: MEDICINAL plants; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MUCUS; Subject Term: ORAL medication; Subject Term: POLYMERASE chain reaction; Subject Term: RATS; Subject Term: SMOKING; Subject Term: STAINS & staining (Microscopy); Subject Term: TRANSCRIPTION factors; Subject Term: TUMOR necrosis factor; Subject Term: WESTERN immunoblotting; Subject Term: PLANT extracts; Subject Term: REVERSE transcriptase polymerase chain reaction; Subject Term: DEXAMETHASONE; Subject Term: INDOLE compounds; Subject Term: FLUORESCENT dyes; Subject Term: CHRONIC bronchitis; Subject Term: PHARMACODYNAMICS; Author-Supplied Keyword: Airway inflammation; Author-Supplied Keyword: Chronic bronchitis; Author-Supplied Keyword: Cigarette smoke; Author-Supplied Keyword: IL-13; Author-Supplied Keyword: MUC5AC; Author-Supplied Keyword: Mucus; Author-Supplied Keyword: Srolo Bzhtang; Author-Supplied Keyword: STAT6; NAICS/Industry Codes: 112990 All Other Animal Production; NAICS/Industry Codes: 325130 Synthetic Dye and Pigment Manufacturing; Number of Pages: 11p; Document Type: ArticleAccession Number: 135255914; Jing, Linde 1,2 Su, Shanshan 3 Zhang, Dejun 4 Li, Zhanqiang 1,2; Email Address: zhanqiang_li@163.com Lu, Dianxiang 1,2; Email Address: ludianxiang@qhu.edu.cn Ge, Rili 1,2; Email Address: geriligao@hotmail.com; Affiliation: 1: Research Center for High Altitude Medicine, Qinghai University, Xining 810001, China 2: Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province, Xining 810001, China 3: Xining Customs District, Key Laboratory of Food Safety Research in Qinghai Province, Xining 810003, China 4: School of Ecological and Environmental Engineering, Qinghai University, Xining 810016; Source Info: May2019, Vol. 235, p424; Subject Term: ANIMAL experimentation; Subject Term: BENZOPYRANS; Subject Term: BRONCHOALVEOLAR lavage; Subject Term: CELLULAR signal transduction; Subject Term: CYTOKINES; Subject Term: ENZYME-linked immunosorbent assay; Subject Term: GENE expression; Subject Term: GLYCOPROTEINS; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: INFLAMMATORY mediators; Subject Term: INTERLEUKINS; Subject Term: LUNGS; Subject Term: MEDICINAL plants; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MUCUS; Subject Term: ORAL medication; Subject Term: POLYMERASE chain reaction; Subject Term: RATS; Subject Term: SMOKING; Subject Term: STAINS & staining (Microscopy); Subject Term: TRANSCRIPTION factors; Subject Term: TUMOR necrosis factor; Subject Term: WESTERN immunoblotting; Subject Term: PLANT extracts; Subject Term: REVERSE transcriptase polymerase chain reaction; Subject Term: DEXAMETHASONE; Subject Term: INDOLE compounds; Subject Term: FLUORESCENT dyes; Subject Term: CHRONIC bronchitis; Subject Term: PHARMACODYNAMICS; Author-Supplied Keyword: Airway inflammation; Author-Supplied Keyword: Chronic bronchitis; Author-Supplied Keyword: Cigarette smoke; Author-Supplied Keyword: IL-13; Author-Supplied Keyword: MUC5AC; Author-Supplied Keyword: Mucus; Author-Supplied Keyword: Srolo Bzhtang; Author-Supplied Keyword: STAT6; NAICS/Industry Codes: 112990 All Other Animal Production; NAICS/Industry Codes: 325130 Synthetic Dye and Pigment Manufacturing; Number of Pages: 11p; Document Type: ArticleAccession Number: 135255914; Jing, Linde 1,2 Su, Shanshan 3 Zhang, Dejun 4 Li, Zhanqiang 1,2; Email Address: zhanqiang_li@163.com Lu, Dianxiang 1,2; Email Address: ludianxiang@qhu.edu.cn Ge, Rili 1,2; Email Address: geriligao@hotmail.com; Affiliation: 1: Research Center for High Altitude Medicine, Qinghai University, Xining 810001, China 2: Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province, Xining 810001, China 3: Xining Customs District, Key Laboratory of Food Safety Research in Qinghai Province, Xining 810003, China 4: School of Ecological and Environmental Engineering, Qinghai University, Xining 810016; Source Info: May2019, Vol. 235, p424; Subject Term: ANIMAL experimentation; Subject Term: BENZOPYRANS; Subject Term: BRONCHOALVEOLAR lavage; Subject Term: CELLULAR signal transduction; Subject Term: CYTOKINES; Subject Term: ENZYME-linked immunosorbent assay; Subject Term: GENE expression; Subject Term: GLYCOPROTEINS; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: INFLAMMATORY mediators; Subject Term: INTERLEUKINS; Subject Term: LUNGS; Subject Term: MEDICINAL plants; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MUCUS; Subject Term: ORAL medication; Subject Term: POLYMERASE chain reaction; Subject Term: RATS; Subject Term: SMOKING; Subject Term: STAINS & staining (Microscopy); Subject Term: TRANSCRIPTION factors; Subject Term: TUMOR necrosis factor; Subject Term: WESTERN immunoblotting; Subject Term: PLANT extracts; Subject Term: REVERSE transcriptase polymerase chain reaction; Subject Term: DEXAMETHASONE; Subject Term: INDOLE compounds; Subject Term: FLUORESCENT dyes; Subject Term: CHRONIC bronchitis; Subject Term: PHARMACODYNAMICS; Author-Supplied Keyword: Airway inflammation; Author-Supplied Keyword: Chronic bronchitis; Author-Supplied Keyword: Cigarette smoke; Author-Supplied Keyword: IL-13; Author-Supplied Keyword: MUC5AC; Author-Supplied Keyword: Mucus; Author-Supplied Keyword: Srolo Bzhtang; Author-Supplied Keyword: STAT6; NAICS/Industry Codes: 112990 All Other Animal Production; NAICS/Industry Codes: 325130 Synthetic Dye and Pigment Manufacturing; Number of Pages: 11p; Document Type: ArticleAccession Number: 135255914; Jing, Linde 1,2 Su, Shanshan 3 Zhang, Dejun 4 Li, Zhanqiang 1,2; Email Address: zhanqiang_li@163.com Lu, Dianxiang 1,2; Email Address: ludianxiang@qhu.edu.cn Ge, Rili 1,2; Email Address: geriligao@hotmail.com; Affiliation: 1: Research Center for High Altitude Medicine, Qinghai University, Xining 810001, China 2: Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province, Xining 810001, China 3: Xining Customs District, Key Laboratory of Food Safety Research in Qinghai Province, Xining 810003, China 4: School of Ecological and Environmental Engineering, Qinghai University, Xining 810016; Source Info: May2019, Vol. 235, p424; Subject Term: ANIMAL experimentation; Subject Term: BENZOPYRANS; Subject Term: BRONCHOALVEOLAR lavage; Subject Term: CELLULAR signal transduction; Subject Term: CYTOKINES; Subject Term: ENZYME-linked immunosorbent assay; Subject Term: GENE expression; Subject Term: GLYCOPROTEINS; Subject Term: IMMUNOHISTOCHEMISTRY; Subject Term: INFLAMMATORY mediators; Subject Term: INTERLEUKINS; Subject Term: LUNGS; Subject Term: MEDICINAL plants; Subject Term: TIBETAN medicine; Subject Term: MESSENGER RNA; Subject Term: MUCUS; Subject Term: ORAL medication; Subject Term: POLYMERASE chain reaction; Subject Term: RATS; Subject Term: SMOKING; Subject Term: STAINS & staining (Microscopy); Subject Term: TRANSCRIPTION factors; Subject Term: TUMOR necrosis factor; Subject Term: WESTERN immunoblotting; Subject Term: PLANT extracts; Subject Term: REVERSE transcriptase polymerase chain reaction; Subject Term: DEXAMETHASONE; Subject Term: INDOLE compounds; Subject Term: FLUORESCENT dyes; Subject Term: CHRONIC bronchitis; Subject Term: PHARMACODYNAMICS; Author-Supplied Keyword: Airway inflammation; Author-Supplied Keyword: Chronic bronchitis; Author-Supplied Keyword: Cigarette smoke; Author-Supplied Keyword: IL-13; Author-Supplied Keyword: MUC5AC; Author-Supplied Keyword: Mucus; Author-Supplied Keyword: Srolo Bzhtang; Author-Supplied Keyword: STAT6; NAICS/Industry Codes: 112990 All Other Animal Production; NAICS/Industry Codes: 325130 Synthetic Dye and Pigment Manufacturing; Number of Pages: 11p; Document Type: Article
Collection:
Himalayan and Tibetan Medicine
Visibility:
Public (group default)
Abstract:
(Show)
Abstract Ethnopharmacological relevance Srolo Bzhtang (SBT), a traditional Tibetan medicine formula, was composed of three herbs, Solms-Laubachia eurycarpa , Bergenia purpurascens , Glycyrrhiza uralensis , and one lac, and was first documented in the ancient Tibetan medical work Four Medical Tantras (rGyud-bzhi) in the eighth century AD. It has been widely used to treat lung "phlegm-heat" syndromes such as chronic bronchitis and chronic obstructive pulmonary disease (COPD). Objective The aim of this study was to evaluate the potential influences of aqueous extract of SBT on airway inflammation and mucus secretion and to reveal the underlying mechanism in a rat model of cigarette smoke (CS)-induced chronic bronchitis (CB). Materials and methods Sixty male Sprague-Dawley rats were randomly divided to six groups: control (room air exposure), model (CS exposure), DEX (CS exposure and 0.2 mg/kg/day dexamethasone), and three SBT (CS exposure and 1.67, 2.50, and 3.34 g/kg/day SBT) groups. DEX and the three doses of SBT were administered by oral gavage every day for eight weeks. Pathological changes and mucus expression in the lung tissue were determined by hematoxylin and eosin (H&E), Alcian blue-periodic acid-Schiff (AB-PAS) and immunohistochemical staining. The levels of cytokines in bronchoalveolar lavage fluid (BALF) were assessed by ELISA. Western blot analysis and qRT-PCR were performed to explore the effects of SBT on the expression of IL-13, STAT6 and MUC5AC. Results Pretreatment with SBT attenuated the TNF-α, IL-8, IL-13 expression levels in BALF and the inflammatory cell infiltration in bronchial walls and peribronchial lung tissue. SBT exhibited a dose-dependent downregulation of MUC5AC expression as assessed by AB-PAS and immunohistochemical staining. The protein and mRNA levels of IL-13, STAT6/p-STAT6 and MUC5AC were also downregulated by SBT preconditioning. Conclusion These results for the first time demonstrated that SBT exhibited protective effects on CS-induced airway inflammation and MUC5AC hypersecretion, which might be related to the downregulation of the IL-13/STAT6 signaling pathway. Graphical abstract fx1 [ABSTRACT FROM AUTHOR]