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[Objectives] By clustering analysis of tissue distribution data of brucine and strychnine in gastric ulcer model rat, the impact of Zuota on tissue distribution of basic components was studied. [Methods] Based on system clustering method of SPSS19.0 statistical analysis software, using inter-group join method and squared Euclidean distance, brucine and strychnine contents of different tissues and organs in non- Zuota group and Zuota group were taken as characteristic variables for clustering analysis, and phylogenetic tree was established. [Results] When clustering distance was 1, (i) taking brucine content as the index, there were three kinds of convergences in non-Zuota group. A1 class: skin, liver, epididymitis and jejunum; A2 class: brain and uterus; A class: testis and muscle. Brucine contents of the three classes showed a A1 < A2 < A. There were two classes of convergences in Zuota group. B1 class : jejunum, epididymis, kidney, brain, skin and uterus; B2 class: muscle and (bottom) submandibular gland. Brucine contents of the two classes showed as B1 < B2. (ii) Taing strychnine content as the index, there were three classes of convergences in non-Zuota group. C1 class: muscle, testicle and oarrum; C2 class: heart and lung; C3 class: uterus and liver. Strychnine contents of the three classes showed a C3 <C1 < C2. There were two kinds of convergences in Zuota group. D1 class : kidney and heart; D2 class : brain tissue and uterus. Strychnine contents of the two classes showed as D1 > D2. [Conclusions] When clustering distance was 1, low-content tissues and orgas firstly clustered, and its toxicological eefect(or pharmacodynamic action)was insignificant, and this kind of tissues and organs were relatively safe. A1 class and A2 class in Zuota group were merged into B1 class, in which liver was replaced by kidney. It iilustrated that Zuota could decline the toxicity of kidney, and enlarged the safe action range of brucine. Kidney and heart in C2 class were clustered into D1 class, and average strychnine content in C2 class was higher than that of D1 class. It could be deduced that Zuota had the effect of protecting heat.

[Objectives] To explore the mechanism for the attenuate-synergistic effect of Zuota to Renqing Mangue, a contrasted study on the tissue distribution of Tibetan medicines Renqing Mangue compatible with Zuota was carried out. [Methods] The SD rats gastric ulcer model were made successfully, and then respectively were given Renqing Mangue compatible with Zuota or not according to the dose of 0.144 g/kg and normal saline by gavage administration, once a day. After 14 d of administration, various organs and tissue were isolated, including brain, heat, liver, spleen, lung, kidney, jejunum, skeletal muscle, fat, skin, submandibular gland, uterus and ovary (or testicle and epididymis). The LC-MS/MS methods were used for determining the contents of brucine and strychnine in various organs and tissue, and significant difference analysis, main target site analysis and clustering analysis were implemented. [Results] Between the Zuota group and non-Zuota group, there was extremely significat diference in the content of brucine in the liver, kidney, muscle, lung, ovary, and heart (P < 0.01), significant difference in the jeeunum (P <0.05), and no significant diference in the epididymis, testis, brain, skin and uterus (P > 0.05); the content of brucine in the submadibular gland, spleen, liver and ovary increased significantly, but significanty reduced in the kidney, muscle, jeeunum, lung and heart. And there was extremely significant diference in the content of strychnine in the liver, kidney, ovary, brain and heat (P <0.01), and no significat difference in the uterus (P > 0.05); the strychnine content increased significantly in the liver, spleen and ovary, and significanty decreased in the kidney, muscle, testis, lung, brain and heart. [Conclusions] Compatibility of Zuota can afect the distribution of brucine and strychnine in some tissue and organs, so a to achieve attenuate-synergistic effect of Zuota to Renqing Mague. [ABSTRACT FROM AUTHOR]