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To study the chemical constituents of the aerial parts of <ce:italic>Codonopsis nervosa</ce:italic> (Chipp.) Nannf. (Campanulaceae), a commonly used Tibetan herb. The constituents were isolated by D-101 porous polymer resin, silica gel, ODS and Sephadex LH-20 column chromatography, and their structures were elucidated on the basis of spectroscopic analysis. 15 compounds were isolated and identified as chrysoeriol (<ce:bold>1</ce:bold>), tricin (<ce:bold>2</ce:bold>), apigenin (<ce:bold>3</ce:bold>), succinic acid <ce:bold>(4)</ce:bold>,<ce:italic>β</ce:italic>-sitosterol-3<ce:italic>-O-β</ce:italic>-D-glucopyranoside (<ce:bold>5</ce:bold>), luteolin (<ce:bold>6</ce:bold>), luteolin-7<ce:italic>-O-β-</ce:italic>D-glucopyranoside (<ce:bold>7</ce:bold>), ethylsyringin (<ce:bold>8</ce:bold>), apigenin-7<ce:italic>-O-β</ce:italic>-D-glucopyranoside (<ce:bold>9</ce:bold>), luteolin-7<ce:italic>-O-β-</ce:italic>D-glucopyranosyl<ce:italic>-</ce:italic>(1→6)-[6′′′-<ce:italic>O</ce:italic>-caffeoyl]-<ce:italic>β</ce:italic>-D-glucopyranoside (<ce:bold>10</ce:bold>), luteolin<ce:italic>-</ce:italic>7<ce:italic>-O-β-</ce:italic>D-gentiobioside (<ce:bold>11</ce:bold>), syringin (<ce:bold>12</ce:bold>), 3<ce:italic>-O-</ce:italic>caffeoylquinic acid (<ce:bold>13</ce:bold>), 5<ce:italic>-O-</ce:italic>caffeoylquinic acid <ce:bold>(14)</ce:bold>, and 4-<ce:italic>O</ce:italic>-(<ce:italic>β-</ce:italic>D-glucopyranosyl)-benzoic acid <ce:bold>(15)</ce:bold>, respectively. Compounds <ce:bold>1-5, 8, 9, 12, 14, 15</ce:bold> were reported from <ce:italic>C. nervosa</ce:italic> for the first time.
A simple, efficient and accurate liquid chromatographic method was established to determine five flavonoid aglycones, 7-hydroxy flavonone, pinocembrin, 2′,4′-dihydroxy chalcone, 2′-hydroxy-4′-methoxy chalcone and pinostrobin in the whole plant powder of <i>Oxytropis falcata</i> Bunge. These five compounds were separated on an Agilent Zorbax Eclipse XDB-C<sub>8</sub> column (150 × 4.6 mm, 5 μm). Mobile phases were composed of water containing 0.1% <i>v/v</i> formic acid and acetonitrile using gradient elution. The established method was validated for linearity, accuracy, precision, limit of detection and quantitation, repeatability and stability.