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Objectives: The objective of this study was to assess the association of trait mindfulness and stress in an urban convenience sample of homeless adults (N = 210). Methods: Associations between trait mindfulness and stress (general perceived stress and chronic urban stress) were assessed using linear regression analyses. Results: Higher trait mindfulness was significantly associated with lower perceived stress (p < .001) and urban stress (p < .001) after adjusting for age, sex, race, marital status, education, income, employment status, and homelessness factors. Conclusions: Higher trait mindfulness may serve as a protective factor against stress among homeless individuals. Future studies should examine the utility of mindfulness-based interventions in improving the health of homeless adults through stress reduction.