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This article reports the development of the 54-item College Chronic Life Stress Survey (CCLSS) and its use in prospective studies of the relationship between chronic stress and psychological distress in college students. Study 1 demonstrated the CCLSS's test-retest reliability and concurrent validity (best friend corroboration of specific items). Study 1 also revealed differential endorsement of specific CCLSS items as a function of gender and year in college. Study 2 cross-sectional and prospective analyses showed that CCLSS chronic stress was a significant predictor of distress. Study 3 cross-sectional analyses showed that the CCLSS effects withstood the statistical control of neuroticism. The findings suggest the value of future research on chronic stress and demonstrate the utility of the CCLSS in studies with college students.