Molecular targeted cancer therapy is a promising strategy to overcome the lack of specificity of anticancer drug. While the binding of c(RGDfK) (cyclic Arginine-Glycine-Aspartic acid-Phenylalanine-Lysine) to αvβ3 over-expressed on tumor cell has been validated, the underlying interaction remains poorly understood. In this work, docking calculation was applied to investigate the interactions between c(RGDfK)/c(RGDfK)-PEG and αvβ3. The calculated results indicated that c(RGDfK) interacted with αvβ3 mainly by electrostatic interaction, stabilization interaction, and hydrophobic interaction. Conjugation of PEG chain to the c(RGDfK) weakened the binding affinity of c(RGDfK) to αvβ3. Accordingly, docetaxel(DTX)-loaded target micelles (c(RGDfK)-PEG-PLA/PEG-PLA/DTX) were designed, characterized and evaluated using HeLa cells. In vitro release studies demonstrated both target and non-target micelles displayed almost the same profiles, which best fit in Ritger-Peppas model. Cellular uptake and MTT studies revealed that the target micelles with the presence of c(RGDfK) were more efficiently taken up by HeLa cells and significantly improved the cytotoxicity compared to that of non-target micelles. Cell inhibition rate of target micelles was improved by 20% after 24h. Our findings suggest that target micelles may be a potential anticancer drug delivery system in the treatment of integrin αvβ3 over-expressed on tumor cell.
PURPOSE: Qigong as a complementary and alternative therapy is often used by cancer patients for symptom management. The aim of this systematic review is to critically evaluate the effectiveness of Qigong on symptom management among cancer patients.METHODS: A systematic search was conducted in the following databases from their inception through May 2016: Cochrane Library, PubMed/MEDLINE, CINAHL, PsycINFO, PEDRO. All controlled clinical trials of Qigong among cancer patients were included. The strength of the evidence was evaluated for all included studies using the Oxford Centre for Evidence-based Medicine Levels of Evidence. The risk of bias was assessed using the Cochrane Collaboration's Tool for Assessing Risk of Bias.
RESULTS: Twenty-two studies including fifteen randomized controlled trials and eight controlled clinical trials examined the efficacy of Qigong in symptom management among patients with various cancers. Results of these studies indicated that symptoms in the Qigong group were significantly improved or there was an observed positive trend from pre-to post-interventions scores for physical symptoms and psychological symptoms.
CONCLUSION: The effectiveness of Qigong as a health practice adopted by cancer patients to manage their symptoms during their cancer journey is not proven, but there are promising results that need further verification in future research.
OBJECTIVE: To explore the effects of traditional Tibetan medicine, Fructus Lonicerae microphyllae (FLM) on phagecytosis and cytokines production of murine macrophages.METHOD: The phagecytosis of murine macrophages was analyzed by neutral red phagecytosis assay. The activities of IL-1 and TNF-alpha were measured by biological methods. The mRNA of TNF-alpha and INF-gamma expressed by macrophages was detected by RT-PCR.
RESULT: The phagecytosis of murine macrophages was significantly enhanced by FLM at a concentration from 1 microg x mL(-1) to 100 microg x mL(-1) and the secretions of IL-1, and TNF-alpha from macrophages were markedly induced by FLM. Meanwhile, FLM also increased the expression of TNF-alpha mRNA and INF-gamma mRHA from macrophages in vitro.
CONCLUSION: FLM could promote phagecytosis and cytokines production of murine macrophages.
The present study is a feasibility study, aimed at evaluating whether a mindfulness-based intervention is acceptable to incarcerated mixed-ethnic Native Hawaiian/Pacific Islander youth, and whether the intervention improves youth’s stress level and results in more skillful response to stress. Measures were collected via 1st-person (self-reports) as well as 3rd-person objective measures (salivary cortisol and SIgA) at pre- and postintervention. Results revealed favorable directions in terms of attenuation of cortisol response and improved SIgA response to stress as well as lower self-report perceived stress (p < .05). Trend level results were also observed for impulsivity, self-regulation, and mindfulness. Collectively, the results suggest that mindfulness-based intervention reduces perceptions of stress and biomarkers of stress and is acceptable among incarcerated ethnic minority youth.
Despite the vast body of research examining the relationship between full-day kindergarten attendance and children's outcomes, little is known about the effects of full-day kindergarten on children with disabilities (i.e., students with 1 of the 13 categories of disabilities recognized under federal law). This study fills this research void by examining whether full-day kindergarten participation predicts differences in achievement and social-emotional outcomes for children with disabilities. Using a national data set of kindergarten students from the 2010-2011 school year (ECLS-K:2011) and employing propensity matching, this study finds that relative to part-day kindergarten (PDK), full-day kindergarten (FDK) attendance is associated with higher achievement scores but also with higher frequencies of internalizing behaviors and lower incidences of self-control at the end of the kindergarten school year. The relationships between FDK attendance and outcomes varied by type of disability classification, such that significant achievement effects emerged only for children with learning and communication disorders. In addition, less time spent on child-initiated activities was associated with higher mathematics scores for children in FDK programs but not for children in PDK programs. Policy implications of the results are discussed.
Mindfulness as focused attention and awareness with acceptance is increasingly being promoted and used as a professional development tool for educators to improve their own as well as students’ well-being, stress, and learning climate. Twenty-six K-12 Hawaii teachers and counselors participated in a three-day mindfulness training course as part of a professional development opportunity. Phenomenological, content analyses of educators’ reflections on personal practices revealed feelings of stress associated with classroom/student management and lack of self-care. Reflections also revealed mindfulness practices helping them to become more aware of their unskillful emotional and mental habit patterns, and how to manage stress. Their reflections on implementing three simple mindfulness lesson plans with students revealed favorable perceptions of mindfulness to improve the lives of their students, the ease as well as challenges of incorporating the practices into the classroom, and the enthusiastic adoption and uptake by the students. Research and policy recommendations as well as implications are discussed, particularly as it relates to current challenges and criticisms of secular mindfulness in education.
Mindfulness as focused attention and awareness with acceptance is increasingly being promoted and used as a professional development tool for educators to improve their own as well as students’ well-being, stress, and learning climate. Twenty-six K-12 Hawaii teachers and counselors participated in a three-day mindfulness training course as part of a professional development opportunity. Phenomenological, content analyses of educators’ reflections on personal practices revealed feelings of stress associated with classroom/student management and lack of self-care. Reflections also revealed mindfulness practices helping them to become more aware of their unskillful emotional and mental habit patterns, and how to manage stress. Their reflections on implementing three simple mindfulness lesson plans with students revealed favorable perceptions of mindfulness to improve the lives of their students, the ease as well as challenges of incorporating the practices into the classroom, and the enthusiastic adoption and uptake by the students. Research and policy recommendations as well as implications are discussed, particularly as it relates to current challenges and criticisms of secular mindfulness in education.
Mindfulness as focused attention and awareness with acceptance is increasingly being promoted and used as a professional development tool for educators to improve their own as well as students’ well-being, stress, and learning climate. Twenty-six K-12 Hawaii teachers and counselors participated in a three-day mindfulness training course as part of a professional development opportunity. Phenomenological, content analyses of educators’ reflections on personal practices revealed feelings of stress associated with classroom/student management and lack of self-care. Reflections also revealed mindfulness practices helping them to become more aware of their unskillful emotional and mental habit patterns, and how to manage stress. Their reflections on implementing three simple mindfulness lesson plans with students revealed favorable perceptions of mindfulness to improve the lives of their students, the ease as well as challenges of incorporating the practices into the classroom, and the enthusiastic adoption and uptake by the students. Research and policy recommendations as well as implications are discussed, particularly as it relates to current challenges and criticisms of secular mindfulness in education.
The article focuses on the study which aims to develop an informed consent process of Tibetan traditional medicine through a randomized placebo-controlled trial in Lhasa, China. The study reveals a process that enables the U.S. and Tibetan research team in meeting their ethical and logistical challenges, and examines the differences between the outcomes. Moreover, a step-by-step process on how participants would react to the research and informed consent process were presented.
OBJECTIVE: To assess the immunosuppressive effect of Artemisia vestita Wall Extract (AV-ext) on mice.METHODS: The proliferative reaction of lymphocyte and the mixed lymphocytes reaction were used to determine the effects of AV-ext on the proliferation of mouse splenocyte in vitro and in vivo; Proliferative reaction of mouse splenocyte was used for detecting the effects of AV-ext on the level of IL-2 secreted by mouse activated splenocyte in vitro. Gelatin enzymogram method and adherence analytical method were employed to disclose the effects of AV-ext on mouse activated T-lymphocytes mobility and adherence.
RESULTS: 1-100 microg/mL AV-ext exerted no obvious toxicity to mouse splenocyte, but it had obvious inhibitory effect on proliferative reaction of mouse splenocyte and mixed lymphocytes reaction induced by ConA. It also had obvious inhibitory effect on the level of IL-2 secreted by mouse activated splenocyte, on the production of MMP-9 by mouse activated T-lymphocytes, and on adherence. 150 mg/kg and 300 mg/kg of AV-ext, given to mouse per os for 7 days, could inhibit the proliferation of splenocyte and the secretion of MMP-9 by activated splenocyte of mouse.
CONCLUSION: AV-ext can inhibit the cellular immune reaction of mouse obviously.
The intent of the current research was to explore the impact of a specific Tibetan Buddhist meditation course containing a lab for applied practice of modern techniques upon psychological well-being in college students. We evaluated the impact of a semester-long undergraduate Tibetan Buddhist meditation course on the psychological well-being of 205 students and assessed whether changes in well-being were mediated by mindfulness. The course was composed of two weekly lectures regarding the tradition and modern applications of meditation, respectively, and a weekly lab in which the students were taught a survey of related modern contemplative techniques to practice. Students were assessed at the beginning, middle, and end of the course, and their time spent practicing the exercises were prospectively recorded. Participants reported statistically significant increases in self-reported mindfulness, self-compassion, and positive coping and significant decreases in self-reported anxiety. Mindfulness was a significant predictor of changes in self-compassion and anxiety. These results suggest that a large lecture course with weekly meditation practice can have a positive impact on the psychological well-being of students and that some of these changes are mediated by mindfulness.
The intent of the current research was to explore the impact of a specific Tibetan Buddhist meditation course containing a lab for applied practice of modern techniques upon psychological well-being in college students. We evaluated the impact of a semester-long undergraduate Tibetan Buddhist meditation course on the psychological well-being of 205 students and assessed whether changes in well-being were mediated by mindfulness. The course was composed of two weekly lectures regarding the tradition and modern applications of meditation, respectively, and a weekly lab in which the students were taught a survey of related modern contemplative techniques to practice. Students were assessed at the beginning, middle, and end of the course, and their time spent practicing the exercises were prospectively recorded. Participants reported statistically significant increases in self-reported mindfulness, self-compassion, and positive coping and significant decreases in self-reported anxiety. Mindfulness was a significant predictor of changes in self-compassion and anxiety. These results suggest that a large lecture course with weekly meditation practice can have a positive impact on the psychological well-being of students and that some of these changes are mediated by mindfulness. (PsycINFO Database Record (c) 2017 APA, all rights reserved)
Procedures of Informed Consent are considered a high priority for international biomedical research. However, informed consent protocols are not necessarily transferable across cultural, national or ethnic groups. Recent debates identify the need for balancing ethical universals with practical and local conditions and paying attention to questions of cultural competence when it comes to the Informed Consent process for clinical biomedical research. This article reports on the results of a two-year effort to establish a culturally appropriate Informed Consent process for biomedical research in the Tibet Autonomous Region in the People's Republic of China. A team of Tibetan and American researchers, physicians, health professionals and medical anthropologists conducted the research. The Informed Consent was specifically for undertaking a triple-blind, double placebo-controlled randomized clinical trial of a Tibetan medicine compared with Misoprostol for reducing postpartum blood loss. The findings suggest greater need for flexibility and cooperation in establishing Informed Consent protocols across cultures and nations.
Our nation's classrooms have become increasingly racially and ethnically diverse. Given these demographic changes, many policymakers and practitioners have expressed the need for increased attention to how teacher diversity might be linked to reducing racial/ethnic differences in teachers' ratings of social-emotional skills for students of color. Using the most recent nationally representative data, we investigated whether kindergarteners have different social-emotional ratings when they had a teacher whose racial/ethnic group was the same as their own. We found that having a teacher of the same race was unrelated to teachers' ratings of children's internalizing problem behaviors, interpersonal skills, approaches to learning, and self-control. However, students whose teachers' race/ethnicity matched their own had more favorable ratings of externalizing behaviors. Results are discussed in terms of implications for school disciplinary policies.
Combinations of multiple meditation practices have been shown to reduce the attrition of telomeres, the protective caps of chromosomes (Carlson et al., 2015). Here, we probed the distinct effects on telomere length (TL) of mindfulness meditation (MM) and loving-kindness meditation (LKM). Midlife adults (N = 142) were randomized to be in a waitlist control condition or to learn either MM or LKM in a 6-week workshop. Telomere length was assessed 2 weeks before the start of the workshops and 3 weeks after their termination. After controlling for appropriate demographic covariates and baseline TL, we found TL decreased significantly in the MM group and the control group, but not in the LKM group. There was also significantly less TL attrition in the LKM group than the control group. The MM group showed changes in TL that were intermediate between the LKM and control groups yet not significantly different from either. Self-reported emotions and practice intensity (duration and frequency) did not mediate these observed group differences. This study is the first to disentangle the effects of LKM and MM on TL and suggests that LKM may buffer telomere attrition.
Objectives:This randomized controlled trial was conducted to examine immune recovery following breast cancer (BC) therapy and evaluate the effect of mindfulness-based stress reduction therapy (MBSR) on immune recovery with emphasis on lymphocyte subsets, T cell activation, and production of T-helper 1 (Th1; interferon [IFN]-γ) and T-helper 2 (Th2; interleukin-4 [IL-4]) cytokines.
Method:
Participants who completed the study consisted of 82 patients diagnosed with Stage 0–III BC, who received lumpectomy and adjuvant radiation ± chemotherapy. Patients were randomized into an MBSR(BC) intervention program or a control (usual care) group. Immune cell measures were assessed at baseline and within 2 weeks after the 6-week intervention. The numbers and percentages of lymphocyte subsets, activated T cells, and Th1 and Th2 cells in peripheral blood samples were determined by immunostaining and flow cytometry.
Results:
Immune subset recovery after cancer treatment showed positive associations with time since treatment completion. The B and natural killer (NK) cells were more susceptible than T cells in being suppressed by cancer treatment. Women who received MBSR(BC) had T cells more readily activated by the mitogen phytohemagglutinin (PHA) and an increase in the Th1/Th2 ratio. Activation was also higher for the MBSR(BC) group if <12 weeks from the end of treatment and women in MBSR(BC) <12 weeks had higher T cell count for CD4+.
Conclusion:
MBSR(BC) promotes a more rapid recovery of functional T cells capable of being activated by a mitogen with the Th1 phenotype, whereas substantial recovery of B and NK cells after completion of cancer treatment appears to occur independent of stress-reducing interventions.
<i>Introduction</i>: To determine the outcomes of vaginal deliveries in three study hospitals in Lhasa, Tibet Autonomous Region (TAR), People's Republic of China (PRC), at high altitude (3650 m). <i>Methods</i>: Prospective observational study of 1121 vaginal deliveries. <i>Results</i>: Pre-eclampsia/gestational hypertension (PE/GH) was the most common maternal complication 18.9% (<i>n</i> = 212), followed by postpartum hemorrhage (blood loss ≥ 500 ml) 13.4%. There were no maternal deaths. Neonatal complications included: low birth weight (10.2%), small for gestational age (13.7%), pre-term delivery (4.1%) and low Apgar (3.7%). There were 11 stillbirths (9.8/1000 live births) and 19 early neonatal deaths (17/1000 live births). <i>Conclusion</i>: This is the largest study of maternal and newborn outcomes in Tibet. It provides information on the outcomes of institutional vaginal births among women delivering infants at high altitude. There was a higher incidence of PE/GH and low birth weight; rates of PPH were not increased compared to those at lower altitudes.
In this article, we contend that an ancient, contemplative practice called mindfulness, often associated with the Buddhist tradition, shares much resonance with the indigenous wisdom of Aloha, the lifestyle and livelihood of Native Hawaiians.
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