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Agroforestry system, as the most promising substitute plantation approach, has been widely regarded as a prominent strategy for mitigating the conflicts between rapid growing population and limited arable land resources. This paper aims to screen the optimal planting pattern for <i>Gentiana rigescens</i> base on the content of gentiopicroside, providing the scientific basis for sustainable supply and application of this plant. Generally, Fourier transform infrared (FTIR) spectroscopy is effective to integrally monitor and reflect the whole constituents of natural materials. FTIR combined with chemometrics was used for distinguishing the <i>G. rigescens</i> from different compound planting models in this research. The result of partial least square discriminant analysis implied that planting year of <i>G. rigescens</i> had a greater impact on the content of gentiopicroside than that of <i>Camellia sinensis</i>. The gentiopicroside content in 1.5- or 2-year-old <i>G. rigescens</i> was higher. Wavelet denoising was effective for the classification. Samples which had higher contents of gentiopicroside were clustered together relatively, while those with lower contents of gentiopicroside were classified into the other large category. Our investigation revealed that <i>G. rigescens</i> can be successfully cultivated with <i>C. sinensis</i>, which met the requirement of the gentiopicroside content recommended by Pharmacopoeia of the People’s Republic of China. That 2-year-old <i>G. rigescens</i> grown with 12-year-old <i>C. sinensis</i> was the optimal compound planting pattern, according this study. The present study provided the optimal compound planting pattern of <i>G. rigescens</i>, which is helpful for improving land-use efficiency and economic returns.
The present study aimed to investigate the effect and underlying mechanisms of the Ruyi Zhenbao pill on neurological function following cerebral ischemia/reperfusion in rats. Male Sprague-Dawley rats underwent middle cerebral artery occlusion following reperfusion. The rats received intragastrically either sodium carboxymethyl cellulose (control and model groups) or Ruyi Zhenbao pill at doses of 0.2, 0.4 or 0.8 g/kg. Neurological function was assessed by cylinder, adhesive and beam-walking tests after 14-day Ruyi Zhenbao pill treatment. Neurogenesis and angiogenesis were detected using immunofluorescence staining. The expression levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assays. Treatment with 0.4 and 0.8 g/kg Ruyi Zhenbao for 14 days significantly improved neurological function, and increased the number of von Willebrand Factor- and neuronal nuclear antigen-positive cells in the ischemic hemisphere of rats. Ruyi Zhenbao pill treatment also significantly enhanced the expression levels of BDNF, NGF and VEGF in the ischemic hemisphere. The results demonstrated that the Ruyi Zhenbao pill improved neurological function following ischemia in rats. The mechanisms of the Ruyi Zhenbao pill are associated with increasing the expression levels of BDNF, NGF and VEGF, and subsequently promoting neurogenesis and angiogenesis in the ischemic zone.