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During the screening of a traditional Chinese folk herb library against HepG2 and Hep3B cell lines, the EtOAc extract from the Tibetan medicine, Caragana tibetica (CT-EtOAc) exhibited potential anti-hepatocellular carcinoma (anti-HCC) activity. HPLC-based activity profiling was performed for targeted identification of anti-HCC activity from CT-EtOAc by MS-directed purification method. CT-EtOAc was separated by time-based fractionation for further anti-HCC bioassay by a semipreparative HPLC column (150 mm × 10 mm i.d., 5 μm) with a single injection of 5 mg. Bioassay-guided and ESIMS-directed large scale purification was performed with a single injection of 400 mg of CT-EtOAc by peak-based fractionation. A 1.4-mm heavy wall micro NMR tube with z-gradient was used to measure one and two dimensional NMR spectra for the minor or trace amounts of components of the extract. Two active compounds could be elucidated as naringenin chalcone (CT-1) and 3-hydroxy-8, 9-dimethoxypterocarpan (CT-2) relevant to anti-HCC effects for the EtOAc extract of C. tibetica rapidly and unambiguously by this protocol.

With the application of artificial fertilizers and pesticides, the quality of the artificial medicinal plants has been found to be in a marked decline. The present paper focuses on the separation of endophytes from wild <i>Angelica sinensis</i> and the effects of endophytes on the growth of <i>A. sinensis</i> and synthesis of secondary metabolites. In this study, the endophytes, namely actinomycetes, fungi, and bacteria, were isolated. Z-ligustilide and ferulic acid were analyzed by HPLC. Our results confirmed that the contents of Z-ligustilide and ferulic acid showed an obvious increase on adding the endophytic fungi, but the additions of the endophytic actinomycetes and bacteria had shown inhibiting effects. Compared with the control group, the contents of Z-ligustilide and ferulic acid in the fungi group increased by 4.12- and 6.53-fold, respectively. This work demonstrates the fact that endophytic fungi can enhance the growth of plants and the production of natural products in <i>A. sinensis.</i>