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An efficient method for the screening and isolation of potent antioxidants from a tibetan medicinal plant by employing online HPLC--DPPH assay combined HSCCC has been successfully established. Six major constituents: boschnaloside (1), alyssonoside (2), leucosceptoside A (3), isoverbascoside (4), leucosceptoside B (5) and verbascoside (6) were isolated and purified from the water extract by HSCCC using ethyl acetate-nbutanol- water as two-phase solvent system. The results indicated that the combination of the online HPLC-- DPPH assay with HSCCC could be suitable for the screening and separation of antioxidant compounds from extract of this plant or other medicine plants. [ABSTRACT FROM AUTHOR]