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Three compounds were isolated from alcoholic extracts of Gentiana tizuensis Franch.. On the basis of spectroscopic methods. Their structures were identified as ursolic acid (1), isooreintin (2), swertiajaponin (3). Among them,compound 2 was isolated from the plant for the first time,compound 3 was isolated from Gentiana for the first time.

The contents of four iridoids ( loganic acid, swertiamarin, gentiopicroside, sweroside) in Gentiana tizuensis Franch. and Gentiana farreri were analyzed by HPLC. The analysis was performed on Econosphere C18 (250 x 4.6 mm, 5 ┬Ám) column, with the solution of 0.5 % acetic acid and methanol as mobile phase, at a flow rate of 1.0 mL/min. The detection wavelength was 254 nm. The results indicated that swertiamarin was not detected in Gentiana tizuensis Franch., and the contents of four iridoids in these two plants were different.