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Two new compounds, deacetylisowortmins A (1) and B (2), were isolated from Talaromyces wortmannii LGT-4. Their structures were established by 1D and 2D NMR spectra, as well as comparison of the experimental and calculated electronic circular dichroism spectra. Monoamine oxidase and acetylcholinesterase inhibitory activities of 1 and 2 were also evaluated.