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Objective: This study aimed to observe curative effect of low load aerobic Yoga-based exercise in cotherapy with inhalation of air negative oxygen ions on occupational patient with cotton pneumoconiosis s. Methods: Select 60 female patients with occupational cotton pneumoconiosis aged between 30 and 50 and randomly divide them into an observation group and a control group with each group consisting of 30 patients. The patients in the control group do low load aerobic Yoga-based exercise for the purpose of improving their physical and respiratory functions, while the patients in the observation group accept co-therapy that combines inhalation of air negative oxygen ions with Yoga exercise. Respectively test and compare the clinical signs and pulmonary function indicators of the two groups of patients at the time of their selection and after 3 months' treatment. Results: after 3 months' treatment, the pulmonary function indicators of the two groups of patients are significantly improved in comparison with their counterparts before the treatment (P<0.05), while the improvement of the pulmonary function and the clinical curative effect of the observation group are more obvious when compared with their counterparts before the treatment or those of the control group, P<0.05. The difference between the improvements shows statistical significance. Conclusion: low load aerobic exercise in the normal air environment can improve lung function of the patient indeed, however, the curative effect is more significant if the exercise is done in the environment with higher air negative oxygen ion content.