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[Obbectives] To study the optimal conditions for extracting procyanidins from Lycium ruthenicum Murr. with sub-critical fluid R134a (1,1,1,2-tetrafluoroethane) in 1 L extraction kettle. [Methods] Taking the extraction rate of procyanidins as an indicator, the influence of preesure, temperature, and extraction time on extraction rate of procyanidins from L. Ruthenicum Murr. was studied by single factor experimental methods and orthogonal array design. [Results] The order of factors affecting extraction rate of procyanidins was extraction temperature k extraction preesure k extraction time. The optimum extraction conditions were as follows % the extraction rate of procyanidins from L. ruthenicum Murr. was the highest with extraction preesure of 1.2 MPa, extraction temperature of 5 0 j and extraction time of 90 min. The content of procyanidins in L. ruthenicum Murr. from different producing areas was determined by vaniilin-HCl method under the optima conditions. [Conclusions] The method has the advantages of easy operation, good selectivity, low extraction temperature and high extraction efficiency, which is suitable for extraction of procyanidins in L. ruthenicum Murr. [ABSTRACT FROM AUTHOR]