Background: Yogic stretching (asana) has been proven to have an effect on salivary human beta‑defensin‑2 (HBD‑2) concentration, which is an antimicrobial peptide and is an inflammatory marker in periodontal disease. Sudarshan Kriya Pranayama (SKP) is a part of yoga which involves rhythmic breathing. Hence, we aim to evaluate the periodontal parameters and to estimate the salivary HBD‑2 level before and after SKP program in periodontitis individuals. Materials and Methods: An interventional study was designed and individuals were divided into three groups: Group I – healthy periodontium, Group II – chronic gingivitis, and Group III – chronic periodontitis. SKP was the interventional tool.The clinical parameters such as plaque index (PI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL), and salivary HBD‑2 level were analyzed at baseline and 90 days after the SKP practice. Enzyme‑linked immunosorbent assay (ELISA) was used to evaluate salivary HBD‑2. McNemar’s Chi‑square, Paired samples t‑test, and one‑way ANOVA were used to analyze the results. P < 0.05 was considered statistically significant.Results: Following the SKP intervention, the clinical parameters such as PI and GI improved significantly in all the groups (P < 0.001); however, Group III showed a significant reduction as compared to the other groups. The mean baseline salivary HBD‑2 levels of Group I, Group II, and Group III were 91.78 ng/µl, 110.22 ng/µl, and 157.63 ng/µl which was further decreased to 95.22 ng/µl, 98.22 ng/µl, and 132.88 ng/µl, respectively, following SKP intervention (P < 0.001). However, Group III had a higher HBD‑2 level at 90th day as compared to other groups. Conclusion:There was an improvement in PI and GI with a decrease in salivary HBD‑2 in chronic periodontitis patients following SKP. Hence, SKP can be considered as an adjunct to treatment modality in patients with periodontal disease.