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Preparative isolation of antioxidative compounds from Dracocephalum heterophyllum using off-line two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography guided by on-line HPLC-DPPH assay.
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
Short Title: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
Format: Journal Article
Publication Year: 2018
Pages: 267 - 274
Sources ID: 93801
Notes: Accession Number: 131404198; Dang, Jun 1,2 Zhang, Li 1,2 Shao, Yun 1,2 Mei, Lijuan 1,2 Liu, Zenggen 1,2 Yue, Huilan 1,2 Wang, Qilan 1,2; Email Address: wql@nwipb.cas.cn Tao, Yanduo 1; Email Address: chemi_ttm_2012@163.com; Affiliation:  1: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, China  2: Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining 810001, China; Source Info: Sep2018, Vol. 1095, p267; Subject Term: HIGH performance liquid chromatography; Subject Term: TIBETAN medicine; Subject Term: ANTIOXIDANTS; Subject Term: ETHYL acetate; Subject Term: POLYPHENOLS; Author-Supplied Keyword: Antioxidant compounds; Author-Supplied Keyword: Dracocephalum heterophyllum; Author-Supplied Keyword: Off-line two-dimensional liquid chromatography; Author-Supplied Keyword: On-line HPLC-DPPH system; NAICS/Industry Codes: 325194 Cyclic Crude, Intermediate, and Gum and Wood Chemical Manufacturing; NAICS/Industry Codes: 325199 All Other Basic Organic Chemical Manufacturing; Number of Pages: 8p; Document Type: Article
Visibility: Public (group default)
Abstract: (Show)
Traditional Tibetan medicine (TTM) has been valuable for the identification of new therapeutic leads. Nevertheless, reports about the chemical constituents of TTM are meager owing to the lack of suitable purification techniques. In this study, an off-line two-dimensional reversed-phase/hydrophilic interaction liquid chromatography (2D RP/HILIC) technique guided by on-line HPLC-DPPH has been established for the isolation of pure antioxidants from the extract of Dracocephalum heterophyllum . According to the chromatographic recognition outcome of the HPLC-DPPH system, the first-dimensional (1D) separation on the Megress C18 preparative column yielded 6 antioxidative fractions (61.4% recovery) from the ethyl acetate fraction (6.1 g). In the second-dimensional (2D) separation, a HILIC XAmide preparative column was employed. In total, 8 antioxidants were isolated from D. heterophyllum with a purity of >95%, which indicated the efficiency of the developed method to prepare antioxidative compounds with high purity from plant extracts. In addition, this method was highly efficient for the preparation of structural analogues of the antioxidative polyphenols and could be applied for the purification of structural analogues from other resources. [ABSTRACT FROM AUTHOR]