A large number of empirical studies have found evidence that neuroticism is related to personality traits, but no one has integrated the relationship between neuroticism and mental health through meta-analysis. As a personality trait, neuroticism reflects the stable tendency of how individuals experience, feel, evaluate negative emotions and make corresponding behavioural responses. By means of meta-analysis, a preliminary dimension of neuroticism is constructed through an open questionnaire and literature review. On this basis, a preliminary neuroticism questionnaire for college students is compiled. The structural model of College Students' neuroticism questionnaire fits well, and has a high correlation with the neuroticism subscale of the simple version of Big Five Personality Questionnaire, which shows that it has a good structural validity. The positive orientation indicators of subjective well-being, life satisfaction and other mental health indicators were also selected. In addition, selfassessment indicators of physical health were selected. It was found that neuroticism was highly correlated with appeal indicators, indicating that the questionnaire of College Students' neuroticism had good validity.