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Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC
Chromatographia Chromatographia : An International Journal for Rapid Communication in Chromatography, Electrophoresis and Associated Techniques
Format: Journal Article
Publication Year: 2016
Pages: 823 - 831
Source ID: shanti-sources-104151
Abstract: A method of using high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) to preparatively separate flavone glucosides and lignan from the crude extracts of Caragana korshinskii has been established for the first time in this study. Five flavone glucosides, including 9 mg of kaempferol 3-O-{β-d-glucopyranosyl(1→2)-[α-l-rhamonopyranosyl(1 → 6)]-β-d-galactopyranoside}, 21 mg of kaempferol 3-O-α-l-rhamnopyranosyl(1→6)-β-d-galactopyranoside-7-O-α-l-rhamnopyranoside, 34 mg of kaempferol 3-O-β-d-galactopyranoside-7-O-α-l-rhamnopyranoside, 27 mg of kaempferol 3-O-β-d-glucopyranosyl-7-O-α-l-rhamnopyranoside, and 14 mg of calycosin 7-O-β-d-glucopyranoside, and one lignan, 16 mg of alangilignoside B, were successfully isolated from 1.8 g of the crude sample through the combination of HSCCC with a two-phase solvent system composed of ethyl acetate-n-butanol-0.5 % formic acid (4:1:5, v/v/v) and semi-preparative RPLC with a mobile phase of methanol-water (20:80, v/v). The purities of the six compounds are all over 95 % as determined by HPLC and the structures are confirmed by the analysis of UV, 1H-NMR, and 13C-NMR and compared with published data.