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Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology
PBA Journal of Pharmaceutical and Biomedical Analysis
Format: Journal Article
Publication Date: Nov 30, 2016
Pages: 182 - 189
Sources ID: 104201
Visibility: Public (group default)
Abstract: (Show)

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• A new protocol of synchronous determination of phenolic acids (PAs) was proposed by RP-HPLC-UV with double-wavelength. • The validated results demonstrated that the proposed method was feasible to determine PAs in plant samples. • The protocol was applied for analysis PAs in Caragana korshinskii Kom. which was mainly rich in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. • Total content of PAs in leaves was the highest compared with that of flowers and bark.
The utilization of Caragana korshinskii Kom. (CK) is currently concentrated on its ecological and fuel functions. Little attention has been devoted to the analysis of their phenolic acid (PA) components. To obtain more data for further utilization of CK, a new analysis protocol was tested to determine PAs synchronously by RP-HPLC-UV with double-wavelength (280 nm and 320 nm) detection. Specifically, separation of PA components was performed on a Hypersil Gold C18 reverse phase column with gradient elution. A four-factor-three-level Box-Behnken design was implemented for optimization of PA extraction. The results demonstrated that CK were rich primarily in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. The total content of PAs in CK leaves was the highest compared with its other parts. The distribution of total flavonoid content of CK was leaves > flowers > bark, while that of the total phenolic content of CK was flowers > leaves > bark.